Products/Services Used | Details | Operation |
---|---|---|
Molecular Biology Reagents> | … For in-vitro aggregation assays, P10 and a total of ten selected P10 variants were purchased from Genscript with purity above 95 % and diluted in DMSO (Sigma-Aldrich code D8418) … | Get A Quote |
The aggregation of epitopes that are also able to bind major histocompatibility complex (MHC) alleles raises questions around the potential connection between the formation of epitope aggregates and their affinities to MHC receptors. We first performed a general bioinformatic assessment over a public dataset of MHC class II epitopes, finding that higher experimental binding correlates with higher aggregation-propensity predictors. We then focused on the case of P10, an epitope used as a vaccine candidate against that aggregates into amyloid fibrils. We used a computational protocol to design variants of the P10 epitope to study the connection between the binding stabilities towards human MHC class II alleles a... More